Cyto3D Live-Dead Assay Kit
The Cyto3D® Live-Dead Assay Kit is a versatile assay, used to determine the live/dead nucleated cells using a quick one-step staining procedure for analysis on a dual-fluorescence system.
The kit is composed of Acridine orange (AO) and Propidium Iodide (PI), both nuclear staining (nucleic acid binding) dyes. AO is permeable to live and dead cells and stains all nucleated cells to generate green fluorescence. PI only penetrates the membranes of nucleated cells with compromised membranes and stains the dead cells to generate red fluorescence.
Due to the quenching, when cells are stained with both AO and PI, all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.
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Description
The Cyto3D® Live-Dead Assay Kit is recommended for viability analysis of cells/organoids cultured in 3D, 2D coating, and on monolayer and works with cells cultured in animal-based ECMs and other hydrogel systems. Dual-fluorescence viability using AO and PI is suitable for staining of 3D and 2D Cell Culture (primary cells and cell lines), Organoids, Spheroids and Stem Cells.
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